E HippoWarehouse alphabet for Shopping Beach Bag Red 10 animal Gym elephant Tote x38cm Classic litres 42cm RRdqxwr E HippoWarehouse alphabet for Shopping Beach Bag Red 10 animal Gym elephant Tote x38cm Classic litres 42cm RRdqxwr
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E litres Shopping 10 elephant x38cm Gym Bag HippoWarehouse Beach animal Tote for Red 42cm Classic alphabet
Classic Gym animal E Beach litres Bag x38cm 10 Shopping Red alphabet 42cm HippoWarehouse elephant for Tote

E HippoWarehouse alphabet for Shopping Beach Bag Red 10 animal Gym elephant Tote x38cm Classic litres 42cm RRdqxwr

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Description:

From a FASTA file and a matching QUAL file, generates a FASTQ file. A minimal FASTQ file omits the redundant sequence label on the quality scores; the quality scores for a sequence are assumed to follow immediately after the sequence with which they are associated. The output FASTQ file will be generated in the specified output directory with the same name as the input FASTA file, suffixed with ‘.fastq’. A FASTQ file will be split into FASTA and QUAL files, and generated in the designated output directory.

Usage: convert_fastaqual_fastq.py [options]

Input Arguments:

Note

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[REQUIRED]

-f, --fasta_file_path
Input FASTA or FASTQ file.

[OPTIONAL]

-q, --qual_file_path
Shopping Beach elephant alphabet Tote animal HippoWarehouse Bag x38cm 42cm Red Classic 10 for E litres Gym Required input QUAL file if converting to FASTQ.
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-o, --output_dir
animal Beach for Red E elephant Shopping Classic 42cm litres 10 alphabet HippoWarehouse Tote Bag x38cm Gym Output directory. Will be created if does not exist. [default: .]
-c, --conversion_type
Bag 42cm Gym Tote elephant 10 HippoWarehouse Shopping Classic for alphabet animal x38cm Red Beach E litres Type of conversion: fastaqual_to_fastq or fastq_to_fastaqual [default: fastaqual_to_fastq]
-a, --ascii_increment
The number to add (subtract if coverting from FASTQ) to the quality score to get the ASCII character (or numeric quality score). [default: 33]
-F, --full_fasta_headers
Include full FASTA headers in output file(s) (as opposed to merely the sequence label). [default: False]
-b, --full_fastq
Include identifiers on quality lines in the FASTQ file (those beginning with a “+”). Irrelevant when converting from FASTQ. [default=False]
-m, Classic alphabet Gym elephant Bag Beach 42cm Red Tote litres E animal for x38cm 10 Shopping HippoWarehouse --multiple_output_files
Create multiple FASTQ files, one for each sample, or create multiple matching FASTA/QUAL for each sample. [default=False]

Output:

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Outputs a complete or minimal FASTQ file, which omits the redundant sequence label on the quality scores, or splits FASTQ file into matching FASTA/QUAL files.

Example:

Using the input files seqs.fna and seqs.qual, generate seqs.fastq in the fastq_files directory:

convert_fastaqual_fastq.py -f seqs.fna -q seqs.qual -o fastq_files/
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Example:

Using input seqs.fastq generate fasta and qual files in fastaqual directory:

convert_fastaqual_fastq.py -c fastq_to_fastaqual -f seqs.fastq -o fastaqual

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